ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer

Abstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithm...

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Main Authors: Yangming Hou, Yingjuan Xu, Dequan Wu
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-90330-3
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spelling doaj-e7afe3020f2e4367a7960ec51de1aacf2021-05-30T11:38:49ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111610.1038/s41598-021-90330-3ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancerYangming Hou0Yingjuan Xu1Dequan Wu2Department of Hepatic Surgery, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Obstetrics and Gynecology, China-Japan Union Hospital, Jilin UniversityDepartment of Hepatic Surgery, The Second Affiliated Hospital of Harbin Medical UniversityAbstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithms were applied to calculate the immune/stromal scores and the proportion of tumor-infiltrating immune cell (TIC) in GC cohort, including 415 cases from The Cancer Genome Atlas (TCGA) database. The differentially expressed genes (DEGs) were screened by Cox proportional hazard regression analysis and protein–protein interaction (PPI) network construction. Then ADAMTS12 was regarded as one of the most predictive factors. Further analysis showed that ADAMTS12 expression was significantly higher in tumor samples and correlated with poor prognosis. Gene Set Enrichment Analysis (GSEA) indicated that in high ADAMTS12 expression group gene sets were mainly enriched in cancer and immune-related activities. In the low ADAMTS12 expression group, the genes were enriched in the oxidative phosphorylation pathway. CIBERSORT analysis for the proportion of TICs revealed that ADAMTS12 expression was positively correlated with Macrophages M0/M1/M2 and negatively correlated with T cells follicular helper. Therefore, ADAMTS12 might be a tumor promoter and responsible for TME status and tumor energy metabolic conversion.https://doi.org/10.1038/s41598-021-90330-3
collection DOAJ
language English
format Article
sources DOAJ
author Yangming Hou
Yingjuan Xu
Dequan Wu
spellingShingle Yangming Hou
Yingjuan Xu
Dequan Wu
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
Scientific Reports
author_facet Yangming Hou
Yingjuan Xu
Dequan Wu
author_sort Yangming Hou
title ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
title_short ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
title_full ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
title_fullStr ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
title_full_unstemmed ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
title_sort adamts12 acts as a tumor microenvironment related cancer promoter in gastric cancer
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-05-01
description Abstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithms were applied to calculate the immune/stromal scores and the proportion of tumor-infiltrating immune cell (TIC) in GC cohort, including 415 cases from The Cancer Genome Atlas (TCGA) database. The differentially expressed genes (DEGs) were screened by Cox proportional hazard regression analysis and protein–protein interaction (PPI) network construction. Then ADAMTS12 was regarded as one of the most predictive factors. Further analysis showed that ADAMTS12 expression was significantly higher in tumor samples and correlated with poor prognosis. Gene Set Enrichment Analysis (GSEA) indicated that in high ADAMTS12 expression group gene sets were mainly enriched in cancer and immune-related activities. In the low ADAMTS12 expression group, the genes were enriched in the oxidative phosphorylation pathway. CIBERSORT analysis for the proportion of TICs revealed that ADAMTS12 expression was positively correlated with Macrophages M0/M1/M2 and negatively correlated with T cells follicular helper. Therefore, ADAMTS12 might be a tumor promoter and responsible for TME status and tumor energy metabolic conversion.
url https://doi.org/10.1038/s41598-021-90330-3
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AT yingjuanxu adamts12actsasatumormicroenvironmentrelatedcancerpromoteringastriccancer
AT dequanwu adamts12actsasatumormicroenvironmentrelatedcancerpromoteringastriccancer
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