ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer
Abstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithm...
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doaj-e7afe3020f2e4367a7960ec51de1aacf2021-05-30T11:38:49ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111610.1038/s41598-021-90330-3ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancerYangming Hou0Yingjuan Xu1Dequan Wu2Department of Hepatic Surgery, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Obstetrics and Gynecology, China-Japan Union Hospital, Jilin UniversityDepartment of Hepatic Surgery, The Second Affiliated Hospital of Harbin Medical UniversityAbstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithms were applied to calculate the immune/stromal scores and the proportion of tumor-infiltrating immune cell (TIC) in GC cohort, including 415 cases from The Cancer Genome Atlas (TCGA) database. The differentially expressed genes (DEGs) were screened by Cox proportional hazard regression analysis and protein–protein interaction (PPI) network construction. Then ADAMTS12 was regarded as one of the most predictive factors. Further analysis showed that ADAMTS12 expression was significantly higher in tumor samples and correlated with poor prognosis. Gene Set Enrichment Analysis (GSEA) indicated that in high ADAMTS12 expression group gene sets were mainly enriched in cancer and immune-related activities. In the low ADAMTS12 expression group, the genes were enriched in the oxidative phosphorylation pathway. CIBERSORT analysis for the proportion of TICs revealed that ADAMTS12 expression was positively correlated with Macrophages M0/M1/M2 and negatively correlated with T cells follicular helper. Therefore, ADAMTS12 might be a tumor promoter and responsible for TME status and tumor energy metabolic conversion.https://doi.org/10.1038/s41598-021-90330-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yangming Hou Yingjuan Xu Dequan Wu |
spellingShingle |
Yangming Hou Yingjuan Xu Dequan Wu ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer Scientific Reports |
author_facet |
Yangming Hou Yingjuan Xu Dequan Wu |
author_sort |
Yangming Hou |
title |
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
title_short |
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
title_full |
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
title_fullStr |
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
title_full_unstemmed |
ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
title_sort |
adamts12 acts as a tumor microenvironment related cancer promoter in gastric cancer |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract The infiltration degree of immune and stromal cells has been shown clinically significant in tumor microenvironment (TME). However, the utility of stromal and immune components in Gastric cancer (GC) has not been investigated in detail. In the present study, ESTIMATE and CIBERSORT algorithms were applied to calculate the immune/stromal scores and the proportion of tumor-infiltrating immune cell (TIC) in GC cohort, including 415 cases from The Cancer Genome Atlas (TCGA) database. The differentially expressed genes (DEGs) were screened by Cox proportional hazard regression analysis and protein–protein interaction (PPI) network construction. Then ADAMTS12 was regarded as one of the most predictive factors. Further analysis showed that ADAMTS12 expression was significantly higher in tumor samples and correlated with poor prognosis. Gene Set Enrichment Analysis (GSEA) indicated that in high ADAMTS12 expression group gene sets were mainly enriched in cancer and immune-related activities. In the low ADAMTS12 expression group, the genes were enriched in the oxidative phosphorylation pathway. CIBERSORT analysis for the proportion of TICs revealed that ADAMTS12 expression was positively correlated with Macrophages M0/M1/M2 and negatively correlated with T cells follicular helper. Therefore, ADAMTS12 might be a tumor promoter and responsible for TME status and tumor energy metabolic conversion. |
url |
https://doi.org/10.1038/s41598-021-90330-3 |
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