Critical Evaluation of Techniques to Detect and Measure Cell Death – Study in a Model of UV Radiation of the Leukaemic Cell Line HL60

The reliability of eight distinct methods (Giemsa staining, trypan blue exclusion, acridine orange/ethidium bromide (AO/EB) double staining for fluorescence microscopy and flow cytometry, propidium iodide (PI) staining, annexin V assay, TUNEL assay and DNA ladder) for detection and quantification of...

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Bibliographic Details
Main Authors: Marina Leite, Margarida Quinta‐Costa, Pedro Simas Leite, José Eduardo Guimarães
Format: Article
Language:English
Published: Hindawi Limited 1999-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/1999/176515