Synergistic Induction of Macrophage Inflammatory Protein-3α/CCL20 Production by Interleukin-17A and Tumor Necrosis Factor-αin Nasal Polyp Fibroblasts

• Main Authors: Manabu Nonaka, MD, PhD, Nozomu Ogihara, MD, Akira Fukumoto, MD, PhD, Atsuko Sakanushi, MD, Kaoru Kusama, MD, Ruby Pawankar, MD, PhD, Toshiaki Yagi, MD, PhD
• Format: Article
• Language: English
• Published: Elsevier 2009-01-01
• Series: World Allergy Organization Journal
• Online Access: http://www.sciencedirect.com/science/article/pii/S193945511930554X
• ISSN: 1939-4551

Background: Accumulation of T cells and immature dendritic cells (DCs) is one of the characteristic features of nasal polyps. However, the question remains why these cells accumulate in nasal polyp tissue. Macrophage inflammatory protein-3α (MIP-3α/CCL20) is a chemokine involved in the migration of T cells and immature DCs into inflammatory tissue sites. Fibroblasts are a rich source of cytokines and chemokines. The objective of this study was to demonstrate the expression of MIP-3α/CCL20 in nasal polyp fibroblasts after stimulation with proinflammatory cytokines such as interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α).Methods: Fibroblast lines were established from nasal polyps. MIP-3α/CCL20 mRNA expression was evaluated by real-time reverse transcription-polymerase chain reaction (real-time RT-PCR). The amount of MIP-3α/CCL20 in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA).Results: IL-17A and TNF-α synergistically induced MIP-3α/CCL20 production by nasal polyp fibroblasts in a dose- and time-dependent manner. This synergy was observed by stimulation with TNF-α plus IL-17A or IL-17F, but not IL-17E.Conclusions: Nasal polyp fibroblasts, by producing MIP-3α/CCL20, may play an important role in the recruitment of T cells and DCs in upper airway inflammatory lesions such as nasal polyps. Keywords: CCR6 ligand, chronic sinusitis, MIP-3α/CCL20, IL-17 family members