| Summary: | Baited lander represents a low impact technique, an alternative to the traditional trawl sampling for collecting data on fish diversity and abundance, especially for threatened species such as Chondrichthyes living in sensitive habitats. In this study, distribution and abundance of cartilaginous fish were compared between two geographic areas, the southern Adriatic Sea and the north-western Ionian Sea, with two low impact sampling gears, an experimental bottom longline and a baited lander. Species diversity was evaluated by applying ecological indices and difference in mean abundances were tested using multivariate analysis. A total of 13 species of cartilaginous fish were collected. Significant differences in the assemblage recorded in the same area using different sampling tools were detected and no significant differences were detected among different areas explored with the same method. Using longline, the most abundant species collected in both areas was <i>Galeus melastomus</i>, while using lander, the most observed species were <i>Dalatias licha</i> in the southern Adriatic Sea and <i>Hexanchus griseus</i> in the north-western Ionian Sea. According to IUCN classification, of the 13 species collected, 2 are near threatened and 5 are threatened. A better governance of sensitive habitats coinciding with the essential fish habitat for these species would ensure them a better conservation status.
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