Genes within the serotonergic system are differentially expressed in human brain

• Main Authors: Craig Ian W, Khan Nadeem, Tichopad Ales, Sugden Karen, D'Souza Ursula M
• Format: Article
• Language: English
• Published: BMC 2009-05-01
• Series: BMC Neuroscience
• Online Access: http://www.biomedcentral.com/1471-2202/10/50

<p>Abstract</p> <p>Background</p> <p>Serotonin is an important neurotransmitter with wide-ranging functions throughout the central nervous system. There is strong evidence to suggest that regulation of serotonergic gene expression might be related to genetic variability, and several studies have focused on understanding the functional effects of specific polymorphisms within these genes on expression levels. However, the combination of genotype together with gender and brain region could have an overall effect on gene expression. In this study, we report expression patterns of five serotonergic genes (<it>TPH1</it>, <it>TPH2, 5-HT2A</it>, <it>5-HT2C</it>, <it>5-HTT</it>) in seven different human post-mortem brain regions (superior frontal gyrus, superior temporal gyrus, striatum, cerebellum, hippocampus, midbrain and thalamus) using TaqMan™ real-time quantitative PCR. In addition, the effect of genotype and gender on their expression levels was determined.</p> <p>Results</p> <p>The data revealed that mRNA from the five genes investigated was detected in all brain regions and showed an overall significant difference in expression levels. Furthermore, the expression of <it>5-HT2C</it>, <it>5-HT2A </it>and <it>TPH2 </it>was found to be significantly different between the various brain regions. However, neither gender nor genotype showed significant effects on the expression levels of any of the genes assayed. Interestingly, <it>TPH1 </it>and <it>TPH2 </it>were expressed in all brain regions similarly except for within the striatum and cerebellum, where <it>TPH1 </it>was expressed at a significantly higher level than <it>TPH2</it>.</p> <p>Conclusion</p> <p>The effect of brain region has a greater influence on serotonergic gene expression than either genotype or gender. These data add to the growing body of evidence that effects of functional polymorphisms on gene expression <it>in vitro </it>are not observed <it>ex vivo</it>, and provide information that will aid in the design of expression studies of the serotonergic gene system within human post-mortem brain.</p>