Directed Evolution of a Homodimeric Laccase from Cerrena unicolor BBP6 by Random Mutagenesis and In Vivo Assembly

Directed Evolution of a Homodimeric Laccase from Cerrena unicolor BBP6 by Random Mutagenesis and In Vivo Assembly

Laccases have great potential for industrial applications due to their green catalytic properties and broad substrate specificities, and various studies have attempted to improve the catalytic performance of these enzymes. Here, to the best of our knowledge, we firstly report the directed evolution...

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Bibliographic Details
Main Authors: Ji Zhang, Fuying Ma, Xiaoyu Zhang, Anli Geng
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:International Journal of Molecular Sciences
Subjects:
Cerrena unicolor
laccase
homodimer
α-factor
low-rate mutation
error-prone PCR
in vivo assembly
enzyme directed evolution
protein modelling
Online Access:http://www.mdpi.com/1422-0067/19/10/2989

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