Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.

Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.

Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. In...

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Main Authors: Raffaella Rossi, Christine Hale, David Goulding, Robert Andrews, Zarah Abdellah, Paul J Fairchild, Gordon Dougan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3532158?pdf=render
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spelling doaj-e75dacfd53b34b94859077746c7d81ea2020-11-24T20:45:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5223210.1371/journal.pone.0052232Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.Raffaella RossiChristine HaleDavid GouldingRobert AndrewsZarah AbdellahPaul J FairchildGordon DouganUsing an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.http://europepmc.org/articles/PMC3532158?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Raffaella Rossi
Christine Hale
David Goulding
Robert Andrews
Zarah Abdellah
Paul J Fairchild
Gordon Dougan
spellingShingle Raffaella Rossi
Christine Hale
David Goulding
Robert Andrews
Zarah Abdellah
Paul J Fairchild
Gordon Dougan
Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
PLoS ONE
author_facet Raffaella Rossi
Christine Hale
David Goulding
Robert Andrews
Zarah Abdellah
Paul J Fairchild
Gordon Dougan
author_sort Raffaella Rossi
title Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
title_short Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
title_full Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
title_fullStr Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
title_full_unstemmed Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
title_sort interaction of salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.
url http://europepmc.org/articles/PMC3532158?pdf=render
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AT christinehale interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT davidgoulding interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
AT robertandrews interactionofsalmonellatyphimuriumwithdendriticcellsderivedfrompluripotentembryonicstemcells
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