Revised selection criteria for candidate restriction enzymes in genome walking.

A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps. These criteria include: step (1) initially choosing the most appropriat...

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Bibliographic Details
Main Authors: Ali Taheri, Stephen J Robinson, Isobel Parkin, Margaret Y Gruber
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3324424?pdf=render