A high-performance liquid chromatography (HPLC) method for determination of chlorogenic acid and emodin in Yinhuang Jiangzhi Tea

• Main Authors: Jie Shen, Xiang-Hui Xu
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-06-01
• Series: Journal of Hainan Medical University
• Subjects: Yinhuang Jiangzhi tea; HPLC; Chlorogenic acid; Emodin
• Online Access: http://www.hnykdxxb.com/PDF/201612/5.pdf

Objective: To establish a high-performance liquid chromatography (HPLC) method for the determination of chlorogenic acid and emodin in Yinhuang Jiangzhi tea. Methods: The contents of chlorogenic acid and emodin in Yinhuang Jiangzhi tea were determined with Diamonsil C18 column by eluting with acetonitrile: 0.4% phosphoric acid in water (13:87) and methanol: 0.1% phosphoricacid in water (80:20), with detection at 327 nm and 254 nm respectively. The flow rate was 1.0 mL/min, and the column temperature was 30℃. Results: The correlation between injection volume and peak area of chlorogenic acid and emodin was Y=17.955X-0.2848 (R=0.9999, 0.041-0.41 μg) and Y=33.975X+186.36 (R=0.9995, 0.0395- 0.395 μg), respectively. The mean recovery of chlorogenic acid and emodin was 100.38% (RSD=2.34%, n=9) and 101.21% (RSD=2.59%, n=9), respectively. Conclusions: The established HPLC method is relatively simple, accurate, and specific, and can be used for the quality control of Yinhuang Jiangzhi tea