Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background

A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluri...

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Main Authors: Qiuting Dai, Weihao Di, Yi Zhang, Shixin Gu, Jian Zhao, Bei Zhang, Ying Wang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001604
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spelling doaj-e6fa0267afc24e30a9c26169b4c527412021-05-30T04:41:30ZengElsevierStem Cell Research1873-50612021-05-0153102314Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic backgroundQiuting Dai0Weihao Di1Yi Zhang2Shixin Gu3Jian Zhao4Bei Zhang5Ying Wang6Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaDepartment of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, ChinaDepartment of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding authors.Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.http://www.sciencedirect.com/science/article/pii/S1873506121001604
collection DOAJ
language English
format Article
sources DOAJ
author Qiuting Dai
Weihao Di
Yi Zhang
Shixin Gu
Jian Zhao
Bei Zhang
Ying Wang
spellingShingle Qiuting Dai
Weihao Di
Yi Zhang
Shixin Gu
Jian Zhao
Bei Zhang
Ying Wang
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
Stem Cell Research
author_facet Qiuting Dai
Weihao Di
Yi Zhang
Shixin Gu
Jian Zhao
Bei Zhang
Ying Wang
author_sort Qiuting Dai
title Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
title_short Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
title_full Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
title_fullStr Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
title_full_unstemmed Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
title_sort generation of a human induced pluripotent stem cell line (siaisi010-a) from a 31-year-old healthy donor with chinese han genetic background
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.
url http://www.sciencedirect.com/science/article/pii/S1873506121001604
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