Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluri...
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doaj-e6fa0267afc24e30a9c26169b4c527412021-05-30T04:41:30ZengElsevierStem Cell Research1873-50612021-05-0153102314Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic backgroundQiuting Dai0Weihao Di1Yi Zhang2Shixin Gu3Jian Zhao4Bei Zhang5Ying Wang6Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaDepartment of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, ChinaDepartment of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding authors.Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.http://www.sciencedirect.com/science/article/pii/S1873506121001604 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiuting Dai Weihao Di Yi Zhang Shixin Gu Jian Zhao Bei Zhang Ying Wang |
spellingShingle |
Qiuting Dai Weihao Di Yi Zhang Shixin Gu Jian Zhao Bei Zhang Ying Wang Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background Stem Cell Research |
author_facet |
Qiuting Dai Weihao Di Yi Zhang Shixin Gu Jian Zhao Bei Zhang Ying Wang |
author_sort |
Qiuting Dai |
title |
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background |
title_short |
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background |
title_full |
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background |
title_fullStr |
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background |
title_full_unstemmed |
Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background |
title_sort |
generation of a human induced pluripotent stem cell line (siaisi010-a) from a 31-year-old healthy donor with chinese han genetic background |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 |
publishDate |
2021-05-01 |
description |
A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies. |
url |
http://www.sciencedirect.com/science/article/pii/S1873506121001604 |
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