Qualitative Indicators of Microbial Cells of Yersinia pestis EV Strain Depending on Their Morphological Traits in Different Temperature Conditions of Manufacturing of Plague Vaccine Preparation

• Main Authors: D. A. Budyka, A. I. Bondarenko, A. A. Fisun, N. V. Abzaeva, G. F. Ivanova, S. E. Gostishcheva, S. M. Rudnev
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2012-08-01
• Series: Проблемы особо опасных инфекций
• Subjects: температурный режим; вакцина чумная живая; параметры качества готового препарата; электронная микроскопия; морфология клетки; лиофилизация; temperature condition; live plague vaccine; quality parameters of stock-produced preparation; electron microscopy; cell morphology; lyophilization
• Online Access: https://journal.microbe.ru/jour/article/view/867
• ISSN: 0370-1069; 2658-719X

The preference of the (21±1) °C temperature for cultivation of the vaccine strain Yersinia pestis EV in the live plague vaccine biomass production to (27±1) °C is experimentally confirmed. An original interpretation of the mechanism of small cells stability that are formed at (21±1) °C has been proposed. This interpretation stipulates a smaller damaging effect of steam molecules during sublimation in the process of lyophilization.