CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia

Abstract Background Leukemia stem cells (LSCs) are responsible for the initiation, progression, and relapse of acute myeloid leukemia (AML). Therefore, a therapeutic strategy targeting LSCs is a potential approach to eradicate AML. In this study, we aimed to identify LSC-specific surface markers and...

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Main Authors: Yongliang Liu, Guiqin Wang, Jiasi Zhang, Xue Chen, Huailong Xu, Gang Heng, Jun Chen, Yongchun Zhao, Jiatao Li, Yuanli Ni, Yingzi Zhang, Juanjuan Shan, Cheng Qian
Format: Article
Language:English
Published: BMC 2021-01-01
Series:Stem Cell Research & Therapy
Subjects:
CD9
Online Access:https://doi.org/10.1186/s13287-021-02155-6
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spelling doaj-e696e57e595e4d75a8f4f6cf2bf6a72b2021-01-31T12:15:59ZengBMCStem Cell Research & Therapy1757-65122021-01-0112111310.1186/s13287-021-02155-6CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemiaYongliang Liu0Guiqin Wang1Jiasi Zhang2Xue Chen3Huailong Xu4Gang Heng5Jun Chen6Yongchun Zhao7Jiatao Li8Yuanli Ni9Yingzi Zhang10Juanjuan Shan11Cheng Qian12Center of Biological Therapy, Southwest Hospital, Army Medical UniversityCenter of Biological Therapy, Southwest Hospital, Army Medical UniversityDepartment of Hematology, Southwest Hospital, Army Medical UniversityDepartment of Hematology, Southwest Hospital, Army Medical UniversityCenter of Biological Therapy, Southwest Hospital, Army Medical UniversityCenter of Biological Therapy, Southwest Hospital, Army Medical UniversityChongqing Institute of Precision Medicine and Biotechnology Co., Ltd.Chongqing Institute of Precision Medicine and Biotechnology Co., Ltd.Center for Precision Medicine of Cancer, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer HospitalCenter for Precision Medicine of Cancer, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer HospitalCenter for Precision Medicine of Cancer, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer HospitalCenter of Biological Therapy, Southwest Hospital, Army Medical UniversityCenter of Biological Therapy, Southwest Hospital, Army Medical UniversityAbstract Background Leukemia stem cells (LSCs) are responsible for the initiation, progression, and relapse of acute myeloid leukemia (AML). Therefore, a therapeutic strategy targeting LSCs is a potential approach to eradicate AML. In this study, we aimed to identify LSC-specific surface markers and uncover the underlying mechanism of AML LSCs. Methods Microarray gene expression data were used to investigate candidate AML-LSC-specific markers. CD9 expression in AML cell lines, patients with AML, and normal donors was evaluated by flow cytometry (FC). The biological characteristics of CD9-positive (CD9+) cells were analyzed by in vitro proliferation, chemotherapeutic drug resistance, migration, and in vivo xenotransplantation assays. The molecular mechanism involved in CD9+ cell function was investigated by gene expression profiling. The effects of alpha-2-macroglobulin (A2M) on CD9+ cells were analyzed with regard to proliferation, drug resistance, and migration. Results CD9, a cell surface protein, was specifically expressed on AML LSCs but barely detected on normal hematopoietic stem cells (HSCs). CD9+ cells exhibit more resistance to chemotherapy drugs and higher migration potential than do CD9-negative (CD9−) cells. More importantly, CD9+ cells possess the ability to reconstitute human AML in immunocompromised mice and promote leukemia growth, suggesting that CD9+ cells define the LSC population. Furthermore, we identified that A2M plays a crucial role in maintaining CD9+ LSC stemness. Knockdown of A2M impairs drug resistance and migration of CD9+ cells. Conclusion Our findings suggest that CD9 is a new biomarker of AML LSCs and is a promising therapeutic target.https://doi.org/10.1186/s13287-021-02155-6Acute myeloid leukemia (AML)Leukemia stem cells (LSCs)CD9Alpha-2-macroglobulin (A2M)Biomarker
collection DOAJ
language English
format Article
sources DOAJ
author Yongliang Liu
Guiqin Wang
Jiasi Zhang
Xue Chen
Huailong Xu
Gang Heng
Jun Chen
Yongchun Zhao
Jiatao Li
Yuanli Ni
Yingzi Zhang
Juanjuan Shan
Cheng Qian
spellingShingle Yongliang Liu
Guiqin Wang
Jiasi Zhang
Xue Chen
Huailong Xu
Gang Heng
Jun Chen
Yongchun Zhao
Jiatao Li
Yuanli Ni
Yingzi Zhang
Juanjuan Shan
Cheng Qian
CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
Stem Cell Research & Therapy
Acute myeloid leukemia (AML)
Leukemia stem cells (LSCs)
CD9
Alpha-2-macroglobulin (A2M)
Biomarker
author_facet Yongliang Liu
Guiqin Wang
Jiasi Zhang
Xue Chen
Huailong Xu
Gang Heng
Jun Chen
Yongchun Zhao
Jiatao Li
Yuanli Ni
Yingzi Zhang
Juanjuan Shan
Cheng Qian
author_sort Yongliang Liu
title CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
title_short CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
title_full CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
title_fullStr CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
title_full_unstemmed CD9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
title_sort cd9, a potential leukemia stem cell marker, regulates drug resistance and leukemia development in acute myeloid leukemia
publisher BMC
series Stem Cell Research & Therapy
issn 1757-6512
publishDate 2021-01-01
description Abstract Background Leukemia stem cells (LSCs) are responsible for the initiation, progression, and relapse of acute myeloid leukemia (AML). Therefore, a therapeutic strategy targeting LSCs is a potential approach to eradicate AML. In this study, we aimed to identify LSC-specific surface markers and uncover the underlying mechanism of AML LSCs. Methods Microarray gene expression data were used to investigate candidate AML-LSC-specific markers. CD9 expression in AML cell lines, patients with AML, and normal donors was evaluated by flow cytometry (FC). The biological characteristics of CD9-positive (CD9+) cells were analyzed by in vitro proliferation, chemotherapeutic drug resistance, migration, and in vivo xenotransplantation assays. The molecular mechanism involved in CD9+ cell function was investigated by gene expression profiling. The effects of alpha-2-macroglobulin (A2M) on CD9+ cells were analyzed with regard to proliferation, drug resistance, and migration. Results CD9, a cell surface protein, was specifically expressed on AML LSCs but barely detected on normal hematopoietic stem cells (HSCs). CD9+ cells exhibit more resistance to chemotherapy drugs and higher migration potential than do CD9-negative (CD9−) cells. More importantly, CD9+ cells possess the ability to reconstitute human AML in immunocompromised mice and promote leukemia growth, suggesting that CD9+ cells define the LSC population. Furthermore, we identified that A2M plays a crucial role in maintaining CD9+ LSC stemness. Knockdown of A2M impairs drug resistance and migration of CD9+ cells. Conclusion Our findings suggest that CD9 is a new biomarker of AML LSCs and is a promising therapeutic target.
topic Acute myeloid leukemia (AML)
Leukemia stem cells (LSCs)
CD9
Alpha-2-macroglobulin (A2M)
Biomarker
url https://doi.org/10.1186/s13287-021-02155-6
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