Characterization of Microbial Communities in Acidified, Sulfur Containing Soils

• Main Authors: Grzegorz Czerwonka, Iwona Konieczna, Paulina Żarnowiec, Artur Zieliński, Agnieszka Malinowska-Gniewosz, Agnieszka Gałuszka, Zdzisław Migaszewski, Wiesław Kaca
• Format: Article
• Language: English
• Published: Exeley Inc. 2017-12-01
• Series: Polish Journal of Microbiology
• Subjects: acidified soil microflora; DNA isolation specific method; soil bacteria; soil biochemical activity
• Online Access: https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/4/pdf/10.5604_01.3001.0010.7043.pdf

Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil micro­bial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified bycultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated Bacillus sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.