Accumulation of Trospectomycin by Strains of Salmonella typhimurium, escherichia coli and Haemophilus influenzae

• Main Authors: S Wong, LE Bryan
• Format: Article
• Language: English
• Published: Hindawi Limited 1990-01-01
• Series: Canadian Journal of Infectious Diseases
• Online Access: http://dx.doi.org/10.1155/1990/258273

Trospectomycin, unlike aminoglycosidic aminocyclitols, is accumulated by a nonsaturable, energy-independent, diffusional process in Salmonella typhimurium, Escherichia coli and Haemophilus influenzae. A deep rough mutant of S typhimurium was more susceptible and accumulated the drug faster, and F porin deficient mutants of E coli were more resistant than parental strains. Trospectomycin likely uses both porin and nonporin pathways to cross the outer membrane. An E coli strain effectively accumulated the drug anaerobically, explaining its anaerobic activity. An H influenzae strain accumulated trospectomycin at concentrations below those for which detectable uptake could be observed with E coli or S typhimurium strains, consistent with greater activity in Haemophilus species.