Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells

• Main Authors: Seung Hyuck Bang, Dong Jun Park, Yang-Hoon Kim, Jiho Min
• Format: Article
• Language: English
• Published: Korean Society of Environmental Helath and Toxicology 2016-05-01
• Series: Environmental Health and Toxicology
• Subjects: Lysosome; Lysosomal membrane proteins; HeLa cell; Melanin; 2-Dimensional electrophoresis
• Online Access: http://www.e-eht.org/upload/pdf/eht-31-e2016009.pdf

Objectives There have been developed to use targeting ability for antimicrobial, anticancerous, gene therapy and cosmetics through analysis of various membrane proteins isolated from cell organelles. Methods It was examined about the lysosomal membrane protein extracted from lysosome isolated from HeLa cell treated by 100 ppm melanin for 24 hours in order to find associated with targeting ability to melanin using by 2-dimensional electrophoresis. Results The result showed 14 up-regulated (1.5-fold) and 13 down-regulated (2.0-fold) spots in relation to melanin exposure. Conclusions It has been found that lysosomal membrane proteins are associated with melanin to decolorize and quantity through cellular activation of lysosome.