Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR

Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR

Abstract Survival of inoculated fungal strains in a new environment plays a critical role in functional performance, but few studies have focused on strain-specific quantitative PCR (qPCR) methods for monitoring beneficial fungi. In this study, the Trichoderma guizhouense strain NJAU 4742 (transform...

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Bibliographic Details
Main Authors: Yang Zhang, Xiang Wang, Guan Pang, Feng Cai, Jian Zhang, Zongzhuan Shen, Rong Li, Qirong Shen
Format: Article
Language:English
Published: SpringerOpen 2019-11-01
Series:AMB Express
Subjects:
Quantitative PCR
Strain-specific primers
Complete genome sequence
Trichoderma
Number detection
Online Access:http://link.springer.com/article/10.1186/s13568-019-0904-4

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