The Usefulness of Active Surveillance Culture of Extended-Spectrum β-Lactamase-producing Escherichia coli in ICU Settings without Outbreak in the Situation of Wide Spread of Sequence Type 131 ESBL-producing E. coli in Community

• Main Authors: Young Ah Kim, Yoon Soo Park, Hyunsoo Kim, Young Hee Seo, Kyungwon Lee
• Format: Article
• Language: English
• Published: Korean Society of Clinical Microbiology 2018-06-01
• Series: Annals of Clinical Microbiology
• Subjects: escherichia coli; extended-spectrum β-lactamase; prevalence; sequence type 131
• Online Access: https://www.acm.or.kr/article_aws/?num=KJCM2018_28
• ISSN: 2288-0585; 2288-6850

Background: In the present study, the prevalence and risk factors for acquisition of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in intensive care unit (ICU) settings without outbreak in the situation of widespread sequence type (ST) 131 ESBL-producing E. coli in a Korean community was investigated.Methods: Consecutive and prospective screening of ESBL-producing E. coli colonization was performed in all patients admitted to surgical or medical ICUs within 48 hours for two months. ESBL genotype was determined based on PCR and sequencing. PCR for O16-ST131/O25-ST131 was performed for all ESBL producers. Clinical information was obtained from a review of electronic medical record to determine the risk factors for ESBL-producing E. coli colonization.Results: The colonization rate of ESBL-producing E. coli at ICU admission was 14.9% (42/281). CTX-M-15 (N=15), CTX-M-14 (N=12), and CTX-M-27 (N=10) were commonly detected using PCR of ESBL genes. Approximately half (45.2%, 19/42) of ESBL producers were ST131 clone with 14 ST131-O25 and 5 ST131- O16. In univariate analysis, independent risk factor for acquisition of ESBL-producing E. coli compared with controls was ICU type (odds ratio, 2.05; P <0.032); however, site of acquisition, previous antibiotic use, and hospital stay were not significant risk factors.Conclusion: In this study, the colonization of ESBLproducing E. coli at ICU admission without outbreak was frequent and it could be an infection source, regardless of acquisition site. We recommend routine use of ASC to control endemic ESBL-producing E.coli considering the wide distribution of ST131-ESBLproducing E. coli in the Korean community. (Ann Clin Microbiol 2018;21:28-35)