O-antigen and virulence profiling of Shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method

O-antigen and virulence profiling of Shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method

Shiga toxin-producing Escherichia coli (STEC) is a leading cause of foodborne illness worldwide. The present study developed the use of DNA microarrays with the ampliPHOX colorimetric method to rapidly detect and genotype STEC strains. A low-density 30-mer oligonucleotide DNA microarray was design...

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Bibliographic Details
Main Authors: Beatriz eQuiñones, Michelle S. Swimley, Koh-Eun eNarm, Ronak N. Patel, Michael B. Cooley, Robert E. Mandrell
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-05-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Escherichia coli
Shiga Toxin
foodborne pathogen
ampliPHOX
STEC
leafy-vegetable production
Online Access:http://journal.frontiersin.org/Journal/10.3389/fcimb.2012.00061/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fcimb.2012.00061/full

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