METAGENOME 16S RRNA GENE ANALYSIS OF THE BLACK SEA MICROBIAL DIVERSITY IN THE REGION OF THE ZMIINIY ISLAND

• Main Authors: О. Є. Боброва, Й. Б. Крістофферсен, В. О. Іваниця
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2015-06-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: чорне море; острів зміїний; морська вода; 16s ррнк аналіз; таксономічна різноманітність; бактерії
• Online Access: http://mbt.onu.edu.ua/article/view/48066

The aim of the study was to determine the marine microbial biodiversity of the Zmiiniy island coastal seawater with the help of metagenomic analysis. Methods. The dualindexing primers were used to amplify v4 region of the 16S rRNA gene. Sequencing was performed on Illumina MiSeq platform. Nucleotide sequences were analyzed by SILVAngs and QIIME pipelines. Results. As the result of metagenomics 16S rRNA gene analysis there were detected about 3200 Operational Taxonomic Units. The main revealed phyla among domain Bacteria were: Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria, Verrucomicrobiota, Planctomycetes, Tenericutes, Fusobacteria, Firmicutes and candidate divisions of uncultivated prokaryotic representatives: SR1, BD1-5, BHI80-139, OP3, OD1, WS3, NPL-UPA2, SHA-109, SM2F11and TM6. As a result of taxonomy analysis lots of bacterial families and genera were identified. The most abundant genera among studied samples were: Marivita, Loktanella, Paracoccus, Pseudoalteromonas, Vibrio, Coraliomargarita, Acholeplasma, Pseudomonas, Phaeobacter, Neptunomonas, Allivibrio, Oceaniserpentilla, Oceanospirillum, Robiginitalea, Acinetobacter, Haliea. Conclusion. The metagenomic 16S rRNA gene analysis allowed evaluating of rich taxonomic prokaryotes diversity in the Zmiiniy island area of the Black Sea surface water. Lots of them were either poorly investigated or uncultivable.