Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes

Abstract Background Insertion of engineered DNA fragments into bacterial vectors is the foundation of recombinant DNA technology, yet existing methods are still laborious, require many steps, depend on specific vector configuration, or require expensive reagents. Results We have developed a method,...

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Bibliographic Details
Main Authors: Matthew D. Fischer, Emmanuel Mgboji, Zhongchi Liu
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Plant Methods
Subjects:
Pyrite
Cloning
Restriction digestion
Single tube
Programmed reaction
Online Access:http://link.springer.com/article/10.1186/s13007-018-0359-7

Internet

http://link.springer.com/article/10.1186/s13007-018-0359-7

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