GERMANIUM COORDINATION COMPOUNDS FOR INCREASING OF α-L-RHAMOSIDASES ACTIVITY

• Main Authors: 1Gudzenko O. V., Varbanets L. D., Seifullina I. I., 2Martsinko E. E., Pirozhok O. V., Chebanenko E. A.
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2019-08-01
• Series: Biotechnologia Acta
• Subjects: germanium coordination compounds; Cryptococcus albidus 1001; Eupenicillium erubescens 248; Penicillium tardum ІМВ F-100074 α-L-rhamnosidases.
• Online Access: http://biotechnology.kiev.ua/images/storage/4_2019/gudzenko_42019.pdf

The aim of the work was to determine the influence of a number of coordination compounds of germanium, in particular cation-anionic type, on the activity of α-L-rhamnosidases of three producers: Cryptococcus albidus, Eupenicillium erubescens and Penicillium tardum ІМВ F-100074. Activity of α-L-rhamnosidases was determined according to Davis method, using naringin as substrate. The specific α-L-rhamnosidase activity of preparations was 12 units/mg of protein for C. аlbidus, 120 units/mg for E. еrubescens and 27 units/mg for P. tardum. Protein concentration in preparations was 0.01 mg/ml. Gernanium compounds were synthesized in alcohol-water solution according to standardized methods. In all compounds, the same bis (citrato) germanate anion is realized; however, the composition of the cation changes: the protonated form of phenanthroline and bipyridine or the complex cations of d-metals. It was established that none of the complex compounds exhibited an inhibitory effect on the activity of the enzymes under study. Maximum activating effect was observed with compound tris(bi-pyridine)nickel(II) bis(citrate)germanate monohydrate in concentration of 0.1% on α-L-rhamnosidases of C. albidus (10-fold), E. еrubescens (2.5-fold) and P. tardum (5-fold). Notably, enzymatic activity increased by 45, 47 and 60% respectively in presences of compound bi-pyridine bis(citrate)germanate dihydrate in same concentration. Hence, it was shown that these two compounds can be used as effectors of studied enzymes.