An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

• Main Authors: Rodrigo Canibano-Fraile, Emma Boertjes, Stela Bozhilova, W.W.M. Pim Pijnappel, Gerben J. Schaaf
• Format: Article
• Language: English
• Published: Elsevier 2021-06-01
• Series: STAR Protocols
• Subjects: Cell isolation; Cell-based Assays; Microscopy; Stem Cells
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166721001891
• ISSN: 2666-1667

Summary: Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.