OPTIMISATION OF Bacillus amyloliquefaciens FE-K1 EXTRACELLULAR PEPTIDASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY

• Main Authors: Fundagül EREM, Mehmet İNAN, Muharrem CERTEL
• Format: Article
• Language: English
• Published: Trakya University 2018-10-01
• Series: Trakya University Journal of Natural Sciences
• Subjects: Bacillus; central composite design; peptidase; response surface methodology
• Online Access: http://dergipark.org.tr/trkjnat/issue/39729/439763?publisher=trakya

In this study, it was aimed to optimise the extracellular peptidase production of Bacillus amyloliquefaciens FE-K1, previously isolated from ropy wholemeal bread, by using response surface methodology (RSM) based on central composite design (CCD). The temperature (20-45°C), initial pH of the enzyme production medium (pH 5-9) and inoculation level (1-5%, v/v) were used as the factors for RSM, and the fermentation time was determined for each trial separately. Results showed that the optimum peptidase production occurred at 33.4°C, pH 6.62 and 2.3% inoculation. It was determined that the fermentation time was only 7h, the crude enzyme had a peptidase activity of 49.17U/mL and a specific activity of 504.77U/mg under the optimised conditions.