The Effect of Adipose-Derived Stem Cell Supernatant on Reducing Sperm Damage During Freezing and Thawing

• Main Authors: F Saghaiti, R Tabatabai, N Kalhor, S Nasri
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2020-10-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: the effect of adipose-derived stem cell supernatant on reducing sperm damage during freezing and thawing
• Online Access: http://armaghanj.yums.ac.ir/article-1-2821-en.html

Background & aim: Infertility and its individual and social problems are one of the main and important issues and concerns of couples and about half of all infertility is related to men. Therefore, the aim of this study was to determine the effect of adipose tissue-derived mesenchymal stem cell supernatant (SPAS) product in reducing sperm damage during freezing and thawing.   Methods: The present descriptive-analytical cross-sectional study was performed in 20 infertile men referred to Qom University Jihad Infertility Treatment Center in 2017. All of these men had asthenospermia. Samples were prepared from these individuals and then pre-freeze measurements such as; Sperm viability, DNA fragmentation, malondialdehyde and oxidative stress were assessed. After that, the stem cells were frozen with the supernatant product and the same previous studies were performed on the defrosted sample. Data were analyzed using one-way analysis of variance.   Results: The results of the present study indicated that sperm survival increased after supernatant defecation. Sperm survival at 25 and 50% concentration was higher than freezing conditions (p <0.05), but no significant difference was seen in DNA doses and oxidative stress in any of the doses (p> 0.05).  The amount of malondialdehyde also changed. The amount of malondialdehyde in SPAS and untreated samples was significantly different from the frozen samples (p <0.05).   Conclusion: Due to the positive effect on sperm and improving their quality after defecation using fat-derived mesenchymal stem cell supernatant, it may be used as a suitable preservative alone or in combination with other supplements. It seems that the increase in sperm viability in the spasm environment was due to the presence of growth factors in this supernatant.