Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model

Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model

In this work, novel bioinspired polyurethane (PU) scaffolds were fabricated via freeze casting for PU-based Pancreatic Ductal Adenocarcinoma (PDAC) model. In order to reproduce the tumour micro-environment that facilitates cellular kinetics, the PU scaffolds were surface modified with extracellular...

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Main Authors: Jingyi Mo, Nathanael Leung, Priyanka Gupta, Bin Zhu, Hui Xing, Jiao Zhang, Eirini Velliou, Tan Sui
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Journal of Materials Research and Technology
Subjects:
Bioinspired polyurethane
Surface functionalisation
Synchrotron X-ray techniques
in situ mechanical testing
Online Access:http://www.sciencedirect.com/science/article/pii/S223878542101022X
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spelling doaj-e4b416d7e2634f1b825ef344c518e66e2021-10-01T04:58:49ZengElsevierJournal of Materials Research and Technology2238-78542021-11-011525072517Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer modelJingyi Mo0Nathanael Leung1Priyanka Gupta2Bin Zhu3Hui Xing4Jiao Zhang5Eirini Velliou6Tan Sui7Bioinspired Materials Group, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK; Corresponding author.Bioinspired Materials Group, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UKBioprocess and Biochemical Engineering Group (BioProChem), Department of Chemical and Process Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UKBioinspired Materials Group, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UKSchool of Materials Science and Engineering, Shang Hai Jiao Tong University, Shang Hai, 200240, People’s Republic of ChinaSchool of Materials Science and Engineering, Shang Hai Jiao Tong University, Shang Hai, 200240, People’s Republic of ChinaBioprocess and Biochemical Engineering Group (BioProChem), Department of Chemical and Process Engineering, University of Surrey, Guildford, Surrey, GU2 7XH, UK; Centre for 3D Models of Health and Disease, Division of Surgery and Interventional Science, University College London, London, W1W 7TY, UKBioinspired Materials Group, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK; Corresponding author.In this work, novel bioinspired polyurethane (PU) scaffolds were fabricated via freeze casting for PU-based Pancreatic Ductal Adenocarcinoma (PDAC) model. In order to reproduce the tumour micro-environment that facilitates cellular kinetics, the PU scaffolds were surface modified with extracellular matrix (ECM) proteins including collagen and fibronectin (Col and FN). Synchrotron-based small- and wide-angle X-ray scattering (SAXS/WAXS) techniques were applied to probe structural evolution during in situ mechanical testing. Strains at macroscopic, nano-, and lattice scales were obtained to investigate the effects of ECM proteins and pancreatic cell activities to PU scaffolds. Significant mechanical strengthening across length scales of PU scaffolds was observed in specimens surface modified by FN. A model of stiffness modulation via enhanced interlamellar recruitment is proposed to explain the multi-scale strengthening mechanisms. Understanding multi-scale deformation mechanisms of a series of PU scaffolds opens an opportunity in developing a novel pancreatic cancer model for studying cancer evolution and predicting outcomes of drug/treatments.http://www.sciencedirect.com/science/article/pii/S223878542101022XBioinspired polyurethaneSurface functionalisationSynchrotron X-ray techniquesin situ mechanical testing
collection DOAJ
language English
format Article
sources DOAJ
author Jingyi Mo
Nathanael Leung
Priyanka Gupta
Bin Zhu
Hui Xing
Jiao Zhang
Eirini Velliou
Tan Sui
spellingShingle Jingyi Mo
Nathanael Leung
Priyanka Gupta
Bin Zhu
Hui Xing
Jiao Zhang
Eirini Velliou
Tan Sui
Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
Journal of Materials Research and Technology
Bioinspired polyurethane
Surface functionalisation
Synchrotron X-ray techniques
in situ mechanical testing
author_facet Jingyi Mo
Nathanael Leung
Priyanka Gupta
Bin Zhu
Hui Xing
Jiao Zhang
Eirini Velliou
Tan Sui
author_sort Jingyi Mo
title Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
title_short Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
title_full Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
title_fullStr Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
title_full_unstemmed Multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
title_sort multi-scale structural and mechanical characterisation in bioinspired polyurethane-based pancreatic cancer model
publisher Elsevier
series Journal of Materials Research and Technology
issn 2238-7854
publishDate 2021-11-01
description In this work, novel bioinspired polyurethane (PU) scaffolds were fabricated via freeze casting for PU-based Pancreatic Ductal Adenocarcinoma (PDAC) model. In order to reproduce the tumour micro-environment that facilitates cellular kinetics, the PU scaffolds were surface modified with extracellular matrix (ECM) proteins including collagen and fibronectin (Col and FN). Synchrotron-based small- and wide-angle X-ray scattering (SAXS/WAXS) techniques were applied to probe structural evolution during in situ mechanical testing. Strains at macroscopic, nano-, and lattice scales were obtained to investigate the effects of ECM proteins and pancreatic cell activities to PU scaffolds. Significant mechanical strengthening across length scales of PU scaffolds was observed in specimens surface modified by FN. A model of stiffness modulation via enhanced interlamellar recruitment is proposed to explain the multi-scale strengthening mechanisms. Understanding multi-scale deformation mechanisms of a series of PU scaffolds opens an opportunity in developing a novel pancreatic cancer model for studying cancer evolution and predicting outcomes of drug/treatments.
topic Bioinspired polyurethane
Surface functionalisation
Synchrotron X-ray techniques
in situ mechanical testing
url http://www.sciencedirect.com/science/article/pii/S223878542101022X
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