Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The...

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Main Authors: Noel Moya, Alireza Shahryari, Ingo Burtscher, Julia Beckenbauer, Mostafa Bakhti, Heiko Lickert
Format: Article
Language:English
Published: Elsevier 2020-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120301756
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spelling doaj-e4a81b5538014a86b6d00bdae1a417242020-11-25T03:48:09ZengElsevierStem Cell Research1873-50612020-07-0146101874Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)Noel Moya0Alireza Shahryari1Ingo Burtscher2Julia Beckenbauer3Mostafa Bakhti4Heiko Lickert5Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Ludwing Maximilian University of Munich, Biology Department, Munich, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Technische Universität München, School of Medicine, Klinikum Rechts der Isar, 81675 München, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, School of Medicine, Klinikum Rechts der Isar, 81675 München, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Corresponding author at: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany.The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro.http://www.sciencedirect.com/science/article/pii/S1873506120301756
collection DOAJ
language English
format Article
sources DOAJ
author Noel Moya
Alireza Shahryari
Ingo Burtscher
Julia Beckenbauer
Mostafa Bakhti
Heiko Lickert
spellingShingle Noel Moya
Alireza Shahryari
Ingo Burtscher
Julia Beckenbauer
Mostafa Bakhti
Heiko Lickert
Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
Stem Cell Research
author_facet Noel Moya
Alireza Shahryari
Ingo Burtscher
Julia Beckenbauer
Mostafa Bakhti
Heiko Lickert
author_sort Noel Moya
title Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
title_short Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
title_full Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
title_fullStr Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
title_full_unstemmed Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)
title_sort generation of a homozygous arx nuclear cfp (arxncfp/ncfp) reporter human ipsc line (hmgui001-a-4)
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-07-01
description The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro.
url http://www.sciencedirect.com/science/article/pii/S1873506120301756
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