UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.

Bibliographic Details
Main Authors: Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Format: Article
Language:English
Published: Nature Publishing Group 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19687-9
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spelling doaj-e4783caa57744ab09ff2474ed8ee0e3c2021-05-11T08:43:44ZengNature Publishing GroupNature Communications2041-17232020-11-0111111010.1038/s41467-020-19687-9UMI-linked consensus sequencing enables phylogenetic analysis of directed evolutionPaul Jannis Zurek0Philipp Knyphausen1Katharina Neufeld2Ahir Pushpanath3Florian Hollfelder4Department of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeJohnson Matthey PlcDepartment of Biochemistry, University of CambridgeThe success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.https://doi.org/10.1038/s41467-020-19687-9
collection DOAJ
language English
format Article
sources DOAJ
author Paul Jannis Zurek
Philipp Knyphausen
Katharina Neufeld
Ahir Pushpanath
Florian Hollfelder
spellingShingle Paul Jannis Zurek
Philipp Knyphausen
Katharina Neufeld
Ahir Pushpanath
Florian Hollfelder
UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
Nature Communications
author_facet Paul Jannis Zurek
Philipp Knyphausen
Katharina Neufeld
Ahir Pushpanath
Florian Hollfelder
author_sort Paul Jannis Zurek
title UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_short UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_full UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_fullStr UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_full_unstemmed UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution
title_sort umi-linked consensus sequencing enables phylogenetic analysis of directed evolution
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-11-01
description The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.
url https://doi.org/10.1038/s41467-020-19687-9
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AT katharinaneufeld umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution
AT ahirpushpanath umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution
AT florianhollfelder umilinkedconsensussequencingenablesphylogeneticanalysisofdirectedevolution
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