Cytology of smears of prints with symptomatic forms of infertility in cows

• Main Authors: A. S. Revunets, S. B. Zarembliuk
• Format: Article
• Language: English
• Published: Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv 2020-10-01
• Series: Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки
• Subjects: symptomatic infertility, cows, vaginal smear, basophiles, neutrophils, parabasal cells, hypofunction of the ovaries, edometritis
• Online Access: https://nvlvet.com.ua/index.php/journal/article/view/4023
• ISSN: 2518-7554; 2518-1327

Many works of domestic and foreign scientists are devoted to the study of etiological factors, pathogenesis, as well as the development of effective methods of diagnosis and therapy for symptomatic infertility of cows, however, insufficient attention is paid to cytological examination of the genital organs.So the goal of our work was to study the cytology of vaginal mucus at the symptomatic form of infertility of cows. The article presents the cytological composition of vaginal mucus from the mucous membrane of the caudal section of the cervix, the lateral surface of the vagina and the dorsal wall of the vaginal vestibule. Research was carried out with 5 groups of cows 4–5 years old cows 6 animals in each, weighing 500–600 kg, with an average productivity of 7500 kg of milk per lactation. The tests were cows were on the 18–24 day after insemination and during the sexual cycle braking, research of the joint course of uterus and hypotension of the ovarian, the presence of persistent corpus luteum and subclinical chronic endometritis, cysts and persistent corpus luteum of the ovarian body. For sub-clinical chronic endometritis, basal cells (32–41 %) are found in wet prep with vaginal mucosa, which are arranged in layers, parabasal (22–6 %) and superficial (5–21 %), but more interstitial (37–66 %), and bare neutrophil nuclei. According to hypofunction of ovaries in vaginal smears, neutrophils, vacuoles in interstitial cell nuclei (44–56 %), basophilic cells (30–43 %), superficial cells (20–21 %), parabasal cells (3–6 %). Basal cell hypofusions were (3–43%), parabasal (3–6 %), interstitial (44–56 %), superficial (20–21 %), and bilateral (12–26 %) (4–5 %) (36–55 %) and (30–33 %). It should be noted that for the functional state of the body, the cells from the cervix are flat. In addition, the drug contains single white blood cells with light cytoplasm without signs of phagocytosis. They are structurally similar to cells of vaginal smear. During the esrtus, the smear is dominated by interstitial and surface cells and white cells, and in the follicular proliferative phase, the number of surface cells increases, while the ovulator phase is characterized by the pronounced maturation of the cells, they are flat and spaced apart.