Microplate Chemiluminescent Assay for DNA Detection Using Apoperoxidase-Oligonucleotide as Capture Conjugate and HRP-Streptavidin Signaling System
A covalent conjugate of horseradish apoperoxidase and amino-containing oligonucleotide was synthesized for the first time. Using the obtained conjugate as a capture reagent chemiluminescent microtiter plate-based assay for detection of 35-mer fragment of hepatitis B virus (HBV) DNA (proof-of-concept...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/4/1289 |