Anti-proliferative activity of ethyl acetate extracts of Tamarix gallica L. grown at different climatic conditions in Algeria

• Main Authors: Fellah Ouroud, Hameurlaine Samir, Gherraf Noureddine, Zellagui Amar, Ali Tahar, Abidi Abdennabi, Altun Muhammed, Demirtas Ibrahim, SahinYaglioglu Ayse
• Format: Article
• Language: English
• Published: Sciendo 2018-12-01
• Series: Acta Scientifica Naturalis
• Subjects: tamarix gallica; geographic origins; ethyl acetate; phenolic; flavonoids; anti-cancer activity
• Online Access: https://doi.org/10.2478/asn-2018-0017
• ISSN: 2367-5144

The aerial parts of T. gallica collected from three different locations (arid, humid and semi-arid) were extracted using ethyl acetate. The crude extracts were subjected to phenolic appraisal and antiproliferative activity using ELISA and xCELLigence assays. The total phenolic and flavonoids were evaluated using appropriate techniques to give a yield of total phenolics ranging between 238.46 and 348.56 mg GAE (Gallic acid equivalent)/g dry weight extract. The flavonoids yield was found to vary from 36.6 to 103.14 mg QE (quercetin equivalent)/g dry weight extract. Moreover, the extracts were tested against rat brain tumor (C6) and human cervix carcinoma (HeLa) cell lines and displayed important differences in activity. These disparities highlighted the effect of climatic factors as quality determinants of secondary metabolites and therefore as a key control of the biological therapeutic effect.