Synergistic Antipseudomonal Effects of Synthetic Peptide AMP38 and Carbapenems

Synergistic Antipseudomonal Effects of Synthetic Peptide AMP38 and Carbapenems

The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentrati...

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Bibliographic Details
Main Authors: Héctor Rudilla, Ester Fusté, Yolanda Cajal, Francesc Rabanal, Teresa Vinuesa, Miguel Viñas
Format: Article
Language:English
Published: MDPI AG 2016-09-01
Series:Molecules
Subjects:
Pseudomonas
antimicrobial peptides
synergism
biofilm eradication
Online Access:http://www.mdpi.com/1420-3049/21/9/1223
Description
Summary:The aim was to explore the antimicrobial activity of a synthetic peptide (AMP38) and its synergy with imipenem against imipenem-resistant Pseudomonas aeruginosa. The main mechanism of imipenem resistance is the loss or alteration of protein OprD. Time-kill and minimal biofilm eradication concentration (MBEC) determinations were carried out by using clinical imipenem-resistant strains. AMP38 was markedly synergistic with imipenem when determined in imipenem-resistant P. aeruginosa. MBEC obtained for the combination of AMP38 and imipenem was of 62.5 μg/mL, whereas the MBEC of each antimicrobial separately was 500 μg/mL. AMP38 should be regarded as a promising antimicrobial to fight MDR P. aeruginosa infections. Moreover, killing effect and antibiofilm activity of AMP38 plus imipenem was much higher than that of colistin plus imipenem.
ISSN:1420-3049

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