Detection of t(8;14) c-myc/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell lymphoma

• Main Authors: Arezoo Kiaei, Habib Onsori, Aylar Alijani, Sasan Andalib, Saeid Ghorbian, Ebrahim Sakhinia
• Format: Article
• Language: English
• Published: Elsevier 2016-12-01
• Series: Hematology/Oncology and Stem Cell Therapy
• Online Access: http://www.sciencedirect.com/science/article/pii/S1658387616300310

Objective/background: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt’s lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90–95% of all chromosomal translocations. This translocation can be found in 2–5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL. Methods: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3). Results: As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene. Conclusion: LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL. Keywords: Diffuse large B-cell lymphoma, Long-distance polymerase chain reaction, Non-Hodgkin lymphoma, Translocation myc/IgH