Challenges in 3D Live Cell Imaging
A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with s...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/8/7/275 |
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doaj-e327b0f34549477f9d87ac05d954d3b62021-07-23T14:01:10ZengMDPI AGPhotonics2304-67322021-07-01827527510.3390/photonics8070275Challenges in 3D Live Cell ImagingHerbert Schneckenburger0Verena Richter1Institute of Applied Research, Aalen University, Beethovenstr. 1, 73430 Aalen, GermanyInstitute of Applied Research, Aalen University, Beethovenstr. 1, 73430 Aalen, GermanyA short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET).https://www.mdpi.com/2304-6732/8/7/2753D cell cultureslight scatteringphototoxicitywide-field microscopylaser scanning microscopystructured illumination |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Herbert Schneckenburger Verena Richter |
| spellingShingle |
Herbert Schneckenburger Verena Richter Challenges in 3D Live Cell Imaging Photonics 3D cell cultures light scattering phototoxicity wide-field microscopy laser scanning microscopy structured illumination |
| author_facet |
Herbert Schneckenburger Verena Richter |
| author_sort |
Herbert Schneckenburger |
| title |
Challenges in 3D Live Cell Imaging |
| title_short |
Challenges in 3D Live Cell Imaging |
| title_full |
Challenges in 3D Live Cell Imaging |
| title_fullStr |
Challenges in 3D Live Cell Imaging |
| title_full_unstemmed |
Challenges in 3D Live Cell Imaging |
| title_sort |
challenges in 3d live cell imaging |
| publisher |
MDPI AG |
| series |
Photonics |
| issn |
2304-6732 |
| publishDate |
2021-07-01 |
| description |
A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET). |
| topic |
3D cell cultures light scattering phototoxicity wide-field microscopy laser scanning microscopy structured illumination |
| url |
https://www.mdpi.com/2304-6732/8/7/275 |
| work_keys_str_mv |
AT herbertschneckenburger challengesin3dlivecellimaging AT verenarichter challengesin3dlivecellimaging |
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