Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells

Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells

Among the beneficial effects of Lactobacillus spp., the common mechanism underlying health protection is believed to be related to antioxidant activity. The antioxidant activities of cell-free suspension (CFS) and strain suspension (SS) of 13 Lactobacillus strains derived from 4 species (L. rhamnosu...

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Main Authors: Jiali Xing, Gang Wang, Qiuxiang Zhang, Xiaoming Liu, Boxing Yin, Dongsheng Fang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen
Format: Article
Language:English
Published: Elsevier 2015-12-01
Series:Journal of Functional Foods
Subjects:
Lactobacillus
Antioxidant activity
Cellular antioxidant assay
Mammal cell
Correlation
Online Access:http://www.sciencedirect.com/science/article/pii/S175646461500434X
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spelling doaj-e2d55bbcf1bc425ea2d4690cbcd783cb2021-04-29T04:45:18ZengElsevierJournal of Functional Foods1756-46462015-12-0119554562Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cellsJiali Xing0Gang Wang1Qiuxiang Zhang2Xiaoming Liu3Boxing Yin4Dongsheng Fang5Jianxin Zhao6Hao Zhang7Yong Q. Chen8Wei Chen9State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China; Corresponding authors. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China. Tel.: +86 51085912155; fax: +86 51085912155.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaKangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou, ChinaKangyuan Dairy Co., Ltd., Yangzhou University, Yangzhou, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China; Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, China; Corresponding authors. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China. Tel.: +86 51085912155; fax: +86 51085912155.Among the beneficial effects of Lactobacillus spp., the common mechanism underlying health protection is believed to be related to antioxidant activity. The antioxidant activities of cell-free suspension (CFS) and strain suspension (SS) of 13 Lactobacillus strains derived from 4 species (L. rhamnosus, L. acidophilus, L. casei, and L. fermenti) were determined. The correlation of CFSs and SSs in different cell lines, as well as the correlation among the three cell models (RAW264.7, Caco-2, and EA.hy926 cell lines), were compared. All CFSs and SSs of lactobacilli showed that they significantly inhibited the production of intracellular reactive oxygen species without creating obvious cytotoxic effects in the three mammal cell lines. These results suggested that RAW264.7, Caco-2, and EA.hy926 cell lines could be used as cell models to assay the cellular antioxidant activity of CFS and SS of lactobacilli. Thus, lactobacilli and by-products are a potential source of natural antioxidants and to reduce oxidative stress.http://www.sciencedirect.com/science/article/pii/S175646461500434XLactobacillusAntioxidant activityCellular antioxidant assayMammal cellCorrelation
collection DOAJ
language English
format Article
sources DOAJ
author Jiali Xing
Gang Wang
Qiuxiang Zhang
Xiaoming Liu
Boxing Yin
Dongsheng Fang
Jianxin Zhao
Hao Zhang
Yong Q. Chen
Wei Chen
spellingShingle Jiali Xing
Gang Wang
Qiuxiang Zhang
Xiaoming Liu
Boxing Yin
Dongsheng Fang
Jianxin Zhao
Hao Zhang
Yong Q. Chen
Wei Chen
Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
Journal of Functional Foods
Lactobacillus
Antioxidant activity
Cellular antioxidant assay
Mammal cell
Correlation
author_facet Jiali Xing
Gang Wang
Qiuxiang Zhang
Xiaoming Liu
Boxing Yin
Dongsheng Fang
Jianxin Zhao
Hao Zhang
Yong Q. Chen
Wei Chen
author_sort Jiali Xing
title Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
title_short Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
title_full Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
title_fullStr Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
title_full_unstemmed Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
title_sort determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2015-12-01
description Among the beneficial effects of Lactobacillus spp., the common mechanism underlying health protection is believed to be related to antioxidant activity. The antioxidant activities of cell-free suspension (CFS) and strain suspension (SS) of 13 Lactobacillus strains derived from 4 species (L. rhamnosus, L. acidophilus, L. casei, and L. fermenti) were determined. The correlation of CFSs and SSs in different cell lines, as well as the correlation among the three cell models (RAW264.7, Caco-2, and EA.hy926 cell lines), were compared. All CFSs and SSs of lactobacilli showed that they significantly inhibited the production of intracellular reactive oxygen species without creating obvious cytotoxic effects in the three mammal cell lines. These results suggested that RAW264.7, Caco-2, and EA.hy926 cell lines could be used as cell models to assay the cellular antioxidant activity of CFS and SS of lactobacilli. Thus, lactobacilli and by-products are a potential source of natural antioxidants and to reduce oxidative stress.
topic Lactobacillus
Antioxidant activity
Cellular antioxidant assay
Mammal cell
Correlation
url http://www.sciencedirect.com/science/article/pii/S175646461500434X
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