CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli

Abstract Background Multiplex control of metabolic pathway genes is essential for maximizing product titers and conversion yields of fuels, chemicals, and pharmaceuticals in metabolic engineering. To achieve this goal, artificial transcriptional regulators, such as clustered regularly interspaced sh...

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Bibliographic Details
Main Authors: Seong Keun Kim, Wonjae Seong, Gui Hwan Han, Dae-Hee Lee, Seung-Goo Lee
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Microbial Cell Factories
Subjects:
CRISPR interference
Escherichia coli
n-Butanol
Multiple gene knockdown
Endogenous gene
Online Access:http://link.springer.com/article/10.1186/s12934-017-0802-x

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