Development of multiplex PCR and multi-color fluorescent in situ hybridization (m-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

Development of multiplex PCR and multi-color fluorescent in situ hybridization (m-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

Abstract Background Several pathogens have been debated to play a role in inflammatory bowel disease (IBD) including Crohn’s disease (CD). None of these pathogens have been investigated together in same clinical samples. We developed a multiplex PCR and multi-color fluorescent in situ hybridization...

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Bibliographic Details
Main Authors: Robert C. Sharp, Ebraheem S. Naser, Karel P. Alcedo, Ahmad Qasem, Latifa S. Abdelli, Saleh A. Naser
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Gut Pathogens
Subjects:
Crohn’s disease
Ulcerative colitis
MAP
m-FISH
Multiplex PCR
Online Access:http://link.springer.com/article/10.1186/s13099-018-0278-1

Internet

http://link.springer.com/article/10.1186/s13099-018-0278-1

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