Quantification bias caused by plasmid DNA conformation in quantitative real-time PCR assay.

Quantification bias caused by plasmid DNA conformation in quantitative real-time PCR assay.

Quantitative real-time PCR (qPCR) is the gold standard for the quantification of specific nucleic acid sequences. However, a serious concern has been revealed in a recent report: supercoiled plasmid standards cause significant over-estimation in qPCR quantification. In this study, we investigated th...

Full description

Bibliographic Details
Main Authors:	Chih-Hui Lin, Yu-Chieh Chen, Tzu-Ming Pan
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2011-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC3237602?pdf=render

Similar Items

Bias of DNA Quantification Caused by Plasmid DNA Conformations in Real-time qPCR Assay
by: Yu-Chieh Chen, et al.
Published: (2010)

Tissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies
by: Joan K Ho, et al.
Published: (2016-01-01)

Identification and quantification of probiotics in the animal feed by PCR-DGGE and real-time quantitative PCR
by: Tzu-Hsing Lin, et al.

Real-time quantitative PCR assay with Taqman® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance
by: S. Chabou, et al.
Published: (2016-09-01)

Development of Quantitative Competitive PCR and Absolute Based Real-Time PCR Assays for Quantification of The Butyrate Producing Bacterium: Butyrivibrio fibrisolvens
by: Mojtaba Tahmoorespur, et al.
Published: (2016-04-01)

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR
by: White, H., et al.
Published: (2015)

Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
by: Varaklioti Agoritsa, et al.
Published: (2010-03-01)

Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays
by: Muradrasoli, Shaman
Published: (2008)

A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
by: Haugland Richard A, et al.
Published: (2008-02-01)

Quantification of Plasma DNA with Cancer by Real-time PCR
by: Chang yu-fang, et al.
Published: (2006)

Comparison of commercial and in-house Real-time PCR assays for quantification of Epstein-Barr virus (EBV) DNA in plasma
by: Pollara Caterina, et al.
Published: (2007-03-01)

Real-time PCR assays for hepatitis B virus DNA quantification may require two different targets
by: Chao Liu, et al.
Published: (2017-05-01)

Analysis of the Level of Plasmid-Derived mRNA in the Presence of Residual Plasmid DNA by Two-Step Quantitative RT-PCR
by: Barbara Ahlemeyer, et al.
Published: (2020-05-01)

DNA and RNA Extraction and Quantitative Real-Time PCR-Based Assays for Biogas Biocenoses in an Interlaboratory Comparison
by: Michael Lebuhn, et al.
Published: (2016-01-01)

Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans
by: Kim van Bergen, et al.
Published: (2021-07-01)

Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)
by: Shengnan He, et al.
Published: (2020-02-01)

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
by: Amin Forootan, et al.
Published: (2017-06-01)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR.
by: Magdalena Plotka, et al.
Published: (2017-01-01)

Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR
by: Martina Beinhauerova, et al.
Published: (2020-07-01)

A Real-Time PCR Assay for the Quantification of Plasmopara viticola Oospores in Grapevine Leaves
by: Melissa Si Ammour, et al.
Published: (2020-08-01)

A real-time PCR assay for quantification of parasite burden in murine models of leishmaniasis
by: Alejandro L. Antonia, et al.
Published: (2018-11-01)

Real-time PCR assays for detection and quantification of early P. falciparum gametocyte stages
by: Amal A. H. Gadalla, et al.
Published: (2021-09-01)

A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana
by: Annegret Ross, et al.
Published: (2016-11-01)

Quantification of Staphylococcus aureus by propidium monoazide in combination with real-time quantitative PCR
by: Meng-Hsuan Lin, et al.
Published: (2015)

Identification and quantification of Bifidobacterium in simulated fermented milk by real-time quantitative PCR
by: Jia-Xin Liu, et al.
Published: (99)

Quantification of bacterial plasmid DNA during chlorophenol biodegradation
by: 張紘偉
Published: (2003)

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes
by: Štrukelj Borut, et al.
Published: (2008-03-01)

Cloning short DNA into plasmids by one‐step PCR
by: Cheng‐Cheng Tao, et al.
Published: (2020-11-01)

Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC
by: Nerurkar Vivek R, et al.
Published: (2006-01-01)

Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris
by: Hadis Jafarian, et al.
Published: (2020-09-01)

Development and validation of a real-time PCR assay for detection and quantification of <it>Tuber magnatum</it> in soil
by: Iotti Mirco, et al.
Published: (2012-06-01)

A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane
by: Yachun Su, et al.
Published: (2013-01-01)

Normalization with Corresponding Naïve Tissue Minimizes Bias Caused by Commercial Reverse Transcription Kits on Quantitative Real-Time PCR Results.
by: Andreas Garcia-Bardon, et al.
Published: (2016-01-01)

Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
by: Phebe Verbrugghe, et al.
Published: (2021-01-01)

Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR
by: Jomehzadeh N, et al.
Published: (2020-05-01)

Real-time quantitative PCR method for detection and quantification of Clostridioides difficile cells and spores
by: Hayden, R.T, et al.
Published: (2022)

Sensitive Forensic DNA Analysis : Application of Pyrosequencing and Real-time PCR Quantification
by: Andréasson, Hanna
Published: (2005)

Development of a Novel Multiplexing Real-Time PCR Machine for DNA Quantification
by: Meng-Hsun Wu, et al.
Published: (2004)

Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis
by: Marre van den Brand, et al.
Published: (2018-04-01)

Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients
by: Elmo W. I. Neuberger, et al.
Published: (2021-06-01)