Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from Assam, North East India

• Main Authors: Irom Manoj Singha, Yelena Kakoty, Bala Gopalan Unni, Jayshree Das, Mohan Chandra Kalita
• Format: Article
• Language: English
• Published: SpringerOpen 2016-06-01
• Series: Journal of Genetic Engineering and Biotechnology
• Subjects: Fusarium; Characterization; ITS; RAPD
• Online Access: http://www.sciencedirect.com/science/article/pii/S1687157X16300191
• ISSN: 1687-157X

Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity. Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40 primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an indispensable method for identification studies.