Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay

Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL) assay. Material...

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Main Authors: Fariba Sharififar, Ali Assadipour, Mohammad Hassan Moshafi, Fahimeh Alishahi, Hossein Mahmoudvand*
Format: Article
Language:English
Published: Lorestan University of Medical Sciences 2017-03-01
Series:Herbal Medicines Journal
Subjects:
Online Access:http://hmj.lums.ac.ir/index.php/hmj/article/view/578
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spelling doaj-e1e1adc622fb4ea29c774383dbd71bed2020-11-24T22:57:21ZengLorestan University of Medical SciencesHerbal Medicines Journal2538-21442538-21442017-03-012110.22087/hmj.v1i2.578508Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity AssayFariba SharififarAli AssadipourMohammad Hassan MoshafiFahimeh AlishahiHossein Mahmoudvand*Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL) assay. Materials and Methods: Essential oils and various extracts of N. sativa were assessed by two methods of disk and solution of BSL. Data analysis was carried out using SPSS statistical package version 17.0 (SPSS Inc., Chicago, IL, 250 USA). Data were processed in probit-analysis program to estimate LC50 values. Results: All of the tested fractions demonstrated more cytotoxicity in the solution method. Petroleum ether and chloroform extract of N. sativa showed the most cytotoxicity with LC50 values 7 and 21 μg/ml respectively; while aqueous and ethanolic had no significant cytotoxicity. Moreover, the GC/MS analysis of the essential oil of N. sativa showed the p-cymene (48.1%), α-thujone (14.38%) and dihydro carveol (9.11%) as the main compounds. Conclusion: These results suggest some limitation for using this spice in diet. Furthermore, this plant could be considered as a source of cytotoxic compounds which should be studied in details.http://hmj.lums.ac.ir/index.php/hmj/article/view/578Nigella sativa, Black cumin, Artemia salina, Cytotoxicity, Essential oil
collection DOAJ
language English
format Article
sources DOAJ
author Fariba Sharififar
Ali Assadipour
Mohammad Hassan Moshafi
Fahimeh Alishahi
Hossein Mahmoudvand*
spellingShingle Fariba Sharififar
Ali Assadipour
Mohammad Hassan Moshafi
Fahimeh Alishahi
Hossein Mahmoudvand*
Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
Herbal Medicines Journal
Nigella sativa, Black cumin, Artemia salina, Cytotoxicity, Essential oil
author_facet Fariba Sharififar
Ali Assadipour
Mohammad Hassan Moshafi
Fahimeh Alishahi
Hossein Mahmoudvand*
author_sort Fariba Sharififar
title Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
title_short Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
title_full Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
title_fullStr Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
title_full_unstemmed Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay
title_sort bioassay screening of the essential oil and various extracts of nigella sativa l. seeds using brine shrimp toxicity assay
publisher Lorestan University of Medical Sciences
series Herbal Medicines Journal
issn 2538-2144
2538-2144
publishDate 2017-03-01
description Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL) assay. Materials and Methods: Essential oils and various extracts of N. sativa were assessed by two methods of disk and solution of BSL. Data analysis was carried out using SPSS statistical package version 17.0 (SPSS Inc., Chicago, IL, 250 USA). Data were processed in probit-analysis program to estimate LC50 values. Results: All of the tested fractions demonstrated more cytotoxicity in the solution method. Petroleum ether and chloroform extract of N. sativa showed the most cytotoxicity with LC50 values 7 and 21 μg/ml respectively; while aqueous and ethanolic had no significant cytotoxicity. Moreover, the GC/MS analysis of the essential oil of N. sativa showed the p-cymene (48.1%), α-thujone (14.38%) and dihydro carveol (9.11%) as the main compounds. Conclusion: These results suggest some limitation for using this spice in diet. Furthermore, this plant could be considered as a source of cytotoxic compounds which should be studied in details.
topic Nigella sativa, Black cumin, Artemia salina, Cytotoxicity, Essential oil
url http://hmj.lums.ac.ir/index.php/hmj/article/view/578
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