Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance

Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance

CRISPR-Cas9 genome engineering has revolutionised high-throughput functional genomic screens. However, recent work has raised concerns regarding the performance of CRISPR-Cas9 screens using TP53 wild-type human cells due to a p53-mediated DNA damage response (DDR) limiting the efficiency of generati...

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Bibliographic Details
Main Authors: Anne Ramsay Bowden, David A Morales-Juarez, Matylda Sczaniecka-Clift, Maria Martin Agudo, Natalia Lukashchuk, John Christopher Thomas, Stephen P Jackson
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-05-01
Series:eLife
Subjects:
CRISPR screening
TP53
DNA damage response
Online Access:https://elifesciences.org/articles/55325

Internet

https://elifesciences.org/articles/55325

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