Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme

Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme

Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS...

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Main Authors: Najeh Maissar Khalil, M. A. M. Mello, Suzelei C França, L. A. A. Oliveira, O M. M. F. Oliveira
Format: Article
Language:English
Published: Universidade Estadual Paulista 2018-05-01
Series:Eclética Química
Online Access:https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/430
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spelling doaj-e1b668cc359148c792fd70fe1e9d7a0f2020-11-24T22:06:50ZengUniversidade Estadual PaulistaEclética Química1678-46182018-05-01313616510.26850/1678-4618eqj.v31.3.2006.p61-65430Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzymeNajeh Maissar Khalil0M. A. M. Mello1Suzelei C França2L. A. A. Oliveira3O M. M. F. Oliveira4Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, 14800-900, Araraquara, SP, Brasil Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, CP 355, 14801-970, Araraquara, SP, BrasilDepartamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, CP 355, 14801-970, Araraquara, SP, BrasilDepartamento de Biotecnologia, UNAERP, 14096-380, Ribeirão Preto, SP, BrasilDepartamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, 14800-900, Araraquara, SP, BrasilDepartamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, CP 355, 14801-970, Araraquara, SP, BrasilPothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperi- od regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxi- dase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for k M and v max ,, respectively, using guaiacol as enzyme substrate.https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/430
collection DOAJ
language English
format Article
sources DOAJ
author Najeh Maissar Khalil
M. A. M. Mello
Suzelei C França
L. A. A. Oliveira
O M. M. F. Oliveira
spellingShingle Najeh Maissar Khalil
M. A. M. Mello
Suzelei C França
L. A. A. Oliveira
O M. M. F. Oliveira
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
Eclética Química
author_facet Najeh Maissar Khalil
M. A. M. Mello
Suzelei C França
L. A. A. Oliveira
O M. M. F. Oliveira
author_sort Najeh Maissar Khalil
title Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_short Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_full Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_fullStr Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_full_unstemmed Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_sort callus cell culture of pothomorphe umbellata (l.) under stress condition leads to high content of peroxidase enzyme
publisher Universidade Estadual Paulista
series Eclética Química
issn 1678-4618
publishDate 2018-05-01
description Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperi- od regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxi- dase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for k M and v max ,, respectively, using guaiacol as enzyme substrate.
url https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/430
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