| Summary: | We assessed the in vitro antimicrobial activity of Peltophorum africanum by means of the agar well and macrodilution methods. The toxicity on a normal human liver cell (Chang liver cell) was determined using the CellTiter-Blue cell viability assay, and the compounds contained in the fractions were identified using GC-MS. Zone diameter of inhibition of the extract ranged from 12.5±0.7 to 32 ± 2.8 mm for bacteria and from 7.5 ± 0.7 to 26.4 ± 3.4 mm for yeast. Marked activity of the extract was observed against Plesiomonas shigelloides ATCC 51903, with MIC and MLC values of 0.15625 and 0.3125 mg/mL, respectively. The extract was both bactericidal (MICindex≤2) and bacteriostatic/fungistatic (MICindex>2) in activity. Lethal dose at 50 (LD50) showed 82.64±1.40 degree of toxicity at 24 hrs, and 95 percentile of cell death dose activity ranged from log 3.12±0.01 to 4.59±0.03. The activity of the eight fractions tested ranged from 1.0±0.5 to 3.7±1.6 mg/mL (IC50) and from 2.1±0.8 to 6.25±0 mg/mL (IC90). The extract was toxic to human Chang liver cell lines.
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