Reduced Culture Temperature Differentially Affects Expression and Biophysical Properties of Monoclonal Antibody Variants

Reduced Culture Temperature Differentially Affects Expression and Biophysical Properties of Monoclonal Antibody Variants

Reduced culture temperature is an increasingly popular practice to improve recombinant protein yields in CHO cells. Recent studies have attributed the enhancement of protein titers at sub-physiological temperatures to increased mRNA levels as well as extended stationary phase. We observed that reduc...

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Bibliographic Details
Main Authors: Megan Mason, Bernadette Sweeney, Katharine Cain, Paul Stephens, Susan T. Sharfstein
Format: Article
Language:English
Published: MDPI AG 2014-08-01
Series:Antibodies
Subjects:
Chinese hamster ovary cells
monoclonal antibodies
protein stability
temperature shift
antibody engineering
Online Access:http://www.mdpi.com/2073-4468/3/3/253

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