Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays

Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays

RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² > 0.99), robust, and specific for the...

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Bibliographic Details
Main Authors: Michele Campos Alves, Priscila Aparecida de Almeida, Hudson Caetano Polonini, Nádia Rezende Barbosa Raposo, Anderson de Oliveira Ferreira, Marcos Antônio Fernandes Brandão
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2014-01-01
Series:Química Nova
Subjects:
Camellia sinensis
quality control
in vitro drug release
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000400024&lng=en&tlng=en
Description
Summary:RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² > 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 µg cm-2. This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane.
ISSN:1678-7064

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