Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling

• Main Authors: Maureira Pablo, Marie Pierre-Yves, Yu Fengxu, Poussier Sylvain, Liu Yihua, Groubatch Frederique, Falanga Aude, Tran Nguyen
• Format: Article
• Language: English
• Published: BMC 2012-11-01
• Series: Journal of Biomedical Science
• Subjects: Chronic myocardial infarction; Tissue engineering; Mesenchymal stem cell; Ventriculoplasty
• Online Access: http://www.jbiomedsci.com/content/19/1/93

<p>Abstract</p> <p>Background</p> <p>Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI).</p> <p>Methods</p> <p>Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry.</p> <p>Results</p> <p>3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 ± 4% <it>vs</it> 49 ± 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p & 0.01). Histology showed an increase of ventricular wall thickness (1.75 ± 0.24 <it>vs</it> 1.35 ± 0.32 mm, p &0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue.</p> <p>Conclusion</p> <p>3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.</p>