Validation of Reference Genes for RT-qPCR Analysis in Noise-Induced Hearing Loss: A Study in Wistar Rat.

The reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) requires adequate normalization in order to ensure accurate results. The use of reference genes is the most common method to normalize RT-qPCR assays; however, many studies have reported that the expression of frequently used...

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Bibliographic Details
Main Authors: Pedro Melgar-Rojas, Juan Carlos Alvarado, Verónica Fuentes-Santamaría, María Cruz Gabaldón-Ull, José M Juiz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4569353?pdf=render

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