Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Abstract Background Glucosamine-6-phosphate N-acetyltransferase (GNA1) is the key enzyme that causes overproduction of N-acetylglucosamine in Bacillus subtilis. Previously, we increased GlcNAc production by promoting the expression of GNA1 from Caenorhabditis elegans (CeGNA1) in an engineered B. sub...

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Bibliographic Details
Main Authors: Wenlong Ma, Yanfeng Liu, Xueqin Lv, Jianghua Li, Guocheng Du, Long Liu
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Microbial Cell Factories
Subjects:
Bacillus subtilis
N-Acetylglucosamine
Glucosamine-6-phosphate N-acetyltransferase
Pyruvate
Overflow
Urease
Online Access:http://link.springer.com/article/10.1186/s12934-018-1049-x

Internet

http://link.springer.com/article/10.1186/s12934-018-1049-x

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