The Establishment of an Assay to Measure DNA Polymerase-Catalyzed Repair of UVB-Induced DNA Damage in Skin Cells and Screening of DNA Polymerase Enhancers from Medicinal Plants

The Establishment of an Assay to Measure DNA Polymerase-Catalyzed Repair of UVB-Induced DNA Damage in Skin Cells and Screening of DNA Polymerase Enhancers from Medicinal Plants

An in vitro assay method was established to measure the activity of cellular DNA polymerases (Pols) in cultured normal human epidermal keratinocytes (NHEKs) by modifying Pol inhibitor activity. Ultraviolet (UV) irradiation enhanced the activity of Pols, especially DNA repair-related Pols, in the cel...

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Bibliographic Details
Main Authors: Sawako Ikeoka, Tatsuo Nakahara, Hiroyasu Iwahashi, Yoshiyuki Mizushina
Format: Article
Language:English
Published: MDPI AG 2016-05-01
Series:International Journal of Molecular Sciences
Subjects:
DNA polymerases
enzyme enhancer
normal human epidermal keratinocytes (NHEK)
ultraviolet B (UVB)
rose myrtle
piceatannol
Online Access:http://www.mdpi.com/1422-0067/17/5/667

Internet

http://www.mdpi.com/1422-0067/17/5/667

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