| Summary: | This paper describes a sensitive spectrophotometric method developed for determination of Ketoconazole (KC) in tablets based on amplification reactions. Ketoconazole was oxidized with periodate, resulting in formation of KC2+ and iodate ions. After masking the excess periodate with molybdate, the iodate was treated with iodide to release iodine. The liberated iodine was transformed to ICl2â species and extracted as ion-pair with rhodamine 6G into toluene for spectrophotometric measurement at 535 nm. A linear calibration graph was obtained between 0.2136 μg/mL and 1.7088 μg/mL of Ketoconazole with a molar absorptivity of 5Ã105 mol·Lâ1 cmâ1. The procedure was successfully applied for the determination of ketoconazole in tablet formulation. Keywords: Ketoconazole, Spectrophotometry, Amplification, Periodate, Oxidation
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