The effect of glycerol in the storage medium on the inhibitory kinetic behavior of glutathione reductase

The effect of glycerol in the storage medium on the inhibitory kinetic behavior of glutathione reductase

In this study we report on the effect of glycerol used in storage buffers, on the inhibitory kinetic behavior of NADP+ on rat kidney glutathione reductase (rkGR) stored at -80°C in ± 10% (v/v) glycerol. At fixed [GSSG] and varied [NADPH], rkGR stored ± glycerol were inhibited competitively by NAD...

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Bibliographic Details
Main Authors: Kulaksiz-Erkmen Gulnihal, Can Betul, Dalmizrak Ozlem, Ogus Hamdi I., Ozer Nazmi
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2012-01-01
Series:Archives of Biological Sciences
Subjects:
Glutathione reductase
glycerol
rat kidney
NADP+
inhibition kinetics
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641201239K.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641201239K.pdf

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